Tsuyako Ōno (February 27, 1934 - January 26, 2021) was a Japanese politician who served two terms in the House of Councillors from 1996 to 2006. Her son was elected to the same legislative body in 2013.
In 1996, Ōno was elected to fill her husband's seat in the Upper House, on behalf of the Liberal Democratic Party; the seat represented Gifu Prefecture.
She won the 2001 election for the same district.
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